. Pluchea lanceolata (DC.) Leaves of P. lanceolata were analyzed for nine chemical characteristics: leaf ash, total phenolics, phosphate, Cu, Zn, Na, K, Mg, and Ca. Many of the effective therapeutic strategies have been derived from ethnopharmacologically used natural products. The samples were kept at 4 C for one hour and then centrifuged at 1 200 g for 20 min at 4 C. These were tested in-vitro against chloroquine-sensitive strain of Plasmodium falciparum NF54 by measuring the parasite specific lactate dehydrogenase activity. In the Indian system of medicine, Pluchea lanceolata is used to treat the neurological disorders. DNA alkaline unwinding assay for monitoring the impact of environmental genotoxins. Omura T, Sato R. The carbon monoxide binding pigment of liver microsomes. 21:1518; 1997. Total extract of PL in semi-solid form was procured from Saiba Industries Pvt. C.B. Side-effects Precautions Interactions Contraindications Overview Pluchea Lanceolatais used for Inflammations and other conditions. Nat Rev Cancer 7(5):357369, Manna P, Das J, Ghosh J, Sil PC (2010) Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IkappaBalpha/NF-kappaB, MAPKs, and mitochondria-dependent pathways: prophylactic role of arjunolic acid. Taxonomy. Weed Science Published online: 21 June 2021 Article Seedling emergence of 144 subalpine meadow plants: effects of phylogeny, life cycle type and seed mass Suzhen Cao, Kun Liu, Guozhen Du, Jerry M. Baskin, Carol C. Baskin, Haiyan Bu and Wei Qi Antiparasitic activities of two sesquiterpenic lactones isolated from Acanthospermum hispidum D.C. Antimalarial efficacy of Pongamia pinnata (L) Pierre against Plasmodium falciparum (3D7 strain) and Plasmodium berghei (ANKA). "coreDisableEcommerce": false, The mission is accomplished through the Society's publications; national and local conferences, meetings and workshops; and through support of teaching initiatives in cooperation with educational institutions. In vitro and field-collected shoots and leaves were tested for response to 2,4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ), for initiation of direct shoot regeneration (DSR), or direct . The amount of dsDNA was obtained from the fluorescence of a sample without any treatment, while ssDNA was determined from the sample that had been boiled for 30 min. Murine malaria was induced by intra-peritoneal injection of Plasmodium berghei infected red blood cells to the male Swiss inbred mice. In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Before Chaturvedi GN, Singh RH. Dep., Lakehead University, Thunder Bay, Ontario, Canada P7B 5E1, Bot. The mobile phase consisted of hexane: ethyl acetate: formic acid (40:20:2.5). volume40,pages 171176 (2004)Cite this article. Evidence from epidemiological, experimental and clinical trial data indicates that a plant based diet can reduce the risk of chronic diseases and reduces toxic effects. 62:239242; 2000. Nanez et al. Borthakur, M.; Dutta, K.; Nath, S. C.; Singh, R. S. Micropropagation of Eclipta alba and Eupatorium adenophorum using a single-step nodal cutting technique. Bot. Batch CBD Full-Spectrum Gummies. 12:5966; 1993. Control and only PL treated groups showed less smearing and an intact band. Plant regeneration from callus cultures derived from primordial leaves of adult Japanese persimmon. Kumar, S., Narula, A., Sharma, M.P. Before Each sample was homogenized with 10 standardized strokes of the pestle to minimize any potential effect on DNA integrity introduced by the homogenization procedure. Bethesda, MD 20894, Web Policies Biochem Pharmacol 84(5):681691, Zhang X, Xiong H, Liu L (2012) Effects of taraxasterol on inflammatory responses in lipopolysaccharide-induced RAW 264.7 macrophages. Orwa JA, Ngeny L, Mwikwabe NM, Ondicho J, Jondiko IJ. Bao-song Tenga, Yan-Hua Lua, Zheng-Tao Wangb, Xin-Yi Taoa, Dong-Zhi Wei. 10. Federal government websites often end in .gov or .mil. Mengel, K.; Kirby, E. A. It is also effective in the treatment of dyspepsia, rheumatoid arthritis and bronchitis (Srivastava et al., 1990). B(a) P is the most commonly studied mutagen with sources of exposure including occupation, diet and tobacco smoke (Phillips, 1999). In the present study, we report the antioxidant and anticlastogenic activity of Pluchea lanceolata (PL), an important medicinal plant, in both in vitro and in vivo model. Study confirms the suitability of Pluchea lanceolata as a candidate for further studies to obtain a prototype for antimalarial medicine. MeSH Hoehne, Luclia Introduction Rasana ( Pluchea lanceolata) is a Sanskrit word means 'a plant having tongue (Rasana) shaped leaves' or the plant which increases ' Rasa ' i.e. Sci. Genomic instability is often measured as the characteristic of cancer. On WB+5.0 mg l1 kinetin, 100% plant regeneration with 140.5 plantlets was obtained. Int J Pharmacogn 34(1):7375, Article This may be related to the more lipophilic substitution (O-methyl group) at the C-3 position in the isorhamnetin molecule. Flavonoidrich diets are reported to have beneficial effects in cardiovascular diseases associated with overproduction of reactive oxygen species. 83:187194; 1995. "coreDisableSocialShare": false, The DNA was visualized using ethidium bromide and photographed. Plant tissue culture and molecular biology. Phytochemistry 43(6):12551260, Article The most potent hexane extract and TxAc were further validated for in-vivo antimalarial and safety evaluation. Aim of the study: Kumar, Virender This mechanism of action is similar to that of aflatoxin, which binds to the N7-position of guanine (Essigmannn, 1977). Amity Institute of Biotechnology, Amity IT University Campus, Sector 125, 201 301, Noida, Uttar Pradesh, India, Centre for Biotechnology, Hamdard University, 110 062, New Delhi, India, Department of Botany, Faculty of Science, Hamdard University, 110 062, New Delhi, India, You can also search for this author in Prochaska HJ, Talalay P. Regulatory mechanisms of monofunctional and bifunctional anticarcinogenic enzyme inducers in murine liver. Formononetin 7-0-glucoside (ononin) an additional growth inhibitor in soils associated with the weed. Results significantly different from corn oil treated group (## Examples of mild side effects that have been reported with Viagra include: mild. The presence of quercetin shown in Figure 3, was determined in the given sample using 25 mg/mL solution in methanol by thin layer chromatography. The reaction mixture consisted of 2 ml phosphate buffer (0.1M, pH 7.4), 0.95 ml hydrogen peroxide (0.019 M) and 0.05 ml PMS in a final volume of 3 ml. p<0.001); PL=Pluchea lanceolata; D1 and D2 = 100 and 200 mg/kg body weight. Effect of Pluchea lanceolata bioactives in LPS-induced neuroinflammation in C6 rat glial cells. Usui, Kenji & Hiern, (Family: Asteraceae) is a rapidly spreading perennial herb, considered valuable for the management of anti-inflammatory disease. doi:10.1016/j.jep.2013.08.003, PubMed The above-mentioned doses of PL were selected based on preliminary studies carried out in our laboratory (data not shown). Plant in January 1991 and continued by In Vitro Cellular & Developmental Biology. Fields cultivated twice a year were designated as Group I, while those cultivated once a year were designated as Group II. 15:473497; 1962. Pluchea lanceolata is an herb employed in Indian folk medicine for malaria like fever but it lacks proper pharmacological intervention. 1998. Frequency of chromosomal aberrations in a subject accidentally exposed to 137Cs in the Gorania(Brazil) radiation accident: Inter comparison among four laboratories. Plant. Antioxidant properties of the methanol extracts from the leaves of Paullinia pinnata. The authors are also grateful to the director of CIMAP for his kind support. Environ. Fitoterapia. This item is part of a JSTOR Collection. For the fluorescence determination of dsDNA, ssDNA and partially unwound DNA (au-DNA), three equal portions of diluted DNA sample were prepared. Learn more about Institutional subscriptions, Akihisa T, Yasukawa K, Oinuma H, Kasahara Y, Yamanouchi S, Takido M, Kumaki K, Tamura T (1996) Triterpene alcohols from the flowers of compositae and their anti-inflammatory effects. Steinmetz KA, Potter JD. https://doi.org/10.1079/IVP2003490. Thirty-five cultivated fields were sampled from seven sites in three different seasons. Pharmacists also advise patients not to drink alcohol with . To evaluate antimalarial and safety profile of Pluchea lanceolata: an in-vitro, in-vivo for its ethnopharmacological validation. Clipboard, Search History, and several other advanced features are temporarily unavailable. Zhong, Z.; Smith, H. G.; Thomas, T. H. In vitro culture of petioles and intact leaves of sugar beet (Beta vulgaris) Plant Growth Regul. Bradford, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Total phenol analysis: automation and comparison with manual methods. 61:481482; 1995a. During DNA fragmentation, DNA damage is estimated in terms of smearing and lack of intact bands. Pluchea lanceolata (Rasana): Chemical and biological potential of Rasayana herb used in traditional system of medicine. Saba; Pande, D.; Iqbal, M.; Srivastava, P. S. Effect of zinc sulphate and copper sulphate on regeneration and lepidine content in Lepidium sativum, L Biol. Trends Neurosci 16(8):323328, Osorio AA, Torres DF, Bedoya LM, Munoz A, Alcami J, Bazzocchi IL (2011) Anti-HIV activity of delta 18-oleane triterpenoids from Cassine xylocarpa. Part of Springer Nature. Planta Med. "corePageComponentUseShareaholicInsteadOfAddThis": true, I. The use of Pluchea lanceolata: It is an effective aphrodisiac. Anal. Materials and methods: Learn more about Institutional subscriptions. Scientific basis of biomarkers and benefits of functional foods for reduction of disease risk. Hostname: page-component-594f858ff7-x2rdm These protective effects of flavonoids are chiefly ascribed to their antioxidant and vasodilator actions (Zenebe, 2001). Eur J Pharm Sci 2, Block ML, Hong JS (2005) Microglia and inflammation-mediated neurodegeneration: multiple triggers with a common mechanism. 8600 Rockville Pike Glia 59(2):242255, Article DNA was isolated and assessed by agarose gel electrophoresis containing ethidiumbromide. Ali, G.; Purohit, M.; Saba; Iqbal, M.; Srivastava, P. S. Morphogenic response and isozymes of Bacopa monniera L. Wettst cultures grown under salt stress. Quercetin (3,5,7,3,4-pentahydroxyflavone) is a flavone with putative ability to prevent cancer and cardiovascular diseases (Jones, 2004). Inclusion in an NLM database does not imply endorsement of, or agreement with, Benzo(a)pyrene (B(a)P), a polycyclic aromatic hydrocarbon, is a byproduct of combustion that causes renal injury and elicits a nephropathic response. Prophylactic administration of PL prior to B (a) P administration significantly decreased the malondialdehyde(MDA), H2O2 and xanthineoxidase (XO) levels at a significance of p<0.001, at both the doses. Induction of antioxidant armory to suppress oxidative stress may be a possible mechanism of PL in modulating B(a)P toxicity. FASEB J: Off Publ Fed Am Soc Exp Biol 13(15):21252137, CAS the herbal drugs will have no or less side effects as it will be applied topically and it will not C.B.Clarke is a wild shrubby member in Asteraceae. Organ Cult. The https:// ensures that you are connecting to the A report about an ongoing trial of the drug from Raleigh-based Sprout Pharmaceuticals for treatment of low sexual desire in women finds in interim results that the so called 'female Viagra' can . Bookshelf Davies, K. L.; Davies, M. S.; Francis, D. Zinc induced vacuolation in root meristematic cells of Festuca rubra L. Plant Cell Environ. C. B. Clarke PLULA, mustard, Brassica juncea (L.) Czern. The oxidized metabolites of carcinogenic compounds are then detoxified by Phase II metabolizing enzymes into the forms that are relatively less toxic and excretable (Bray, 1993; Zunino, 1989). The yield of quercetin in culture varied with stages of differentiation. Table 1 depicts the effect of PL pretreatment on B(a)P induced alterations in reduced glutathione(GSH) content and its redox cycle. Effect of pretreatment of PL extract on antioxidant enzymes on B(a)p administration in kidney of Swiss albino mice. The anticarcinogenic effect or function of natural compounds might be attributed to a combination of their cytoprotective effect on normal cells and their cytotoxic effect on preneoplastic and/or neoplastic cells. Tissue glutathione, nutrition and oxidative stress. An increased number of micronuclei in PCE in comparison with the control group indicate that B(a)P produces chromosomal damage in erythrocytes of bone marrow and this damage is associated with the appearance and/or progression of tumors with adverse reproductive and developmental outcomes (Krishna & Hayashi, 2000). There is a probability that benzo[a]pyrene diol epoxide inactivates the tumor suppression ability in certain cells, leading to cancer. Planta Med 77(12):13411341, Article Kobayashi, Katsuichiro They counteract with free radicals through their antioxidant mechanisms. New Delhi: Council of Scientific and Industrial Research; 1998. Khan TH, Prasad L, Anuradha, Sultana S. Soy isoflavones inhibit the genotoxicity of benzo(a)pyrene in Swiss albino mice. Pluchea lanceolata (DC.) Bellinder, Robin R. XII. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Tiwati, V.; Tiwari, K. N.; Singh, B. D. Comparative studies of cytokinins on in vitro propagation of Bacopa monnierd. 72:248254; 1976. Npoje s vysokm obsahom antioxidantov, ako s vitamny C a E, preukzatene zlepuj erektiln funkciu tm, e brnia pokodeniu buniek, produkujcich oxid dusnat," hovor Pearlmanov. Experimental studies on the anti arthritic effect of certain indigenous drugs. Phytomorphology 47:97106; 1997. Disruption of glomerular cellcell and cellmatrix interactions in hydrocarbon nephropathy. Antimalarial and safety evaluation of extracts from Toddalia asiatica (L) Lam. New Phytol. In Vitro Cellular & Developmental Biology. Weed-infested topsoil and subsoil were analyzed for pH, electrical conductivity, organic carbon, chloride, bicarbonate, total carbonate, phosphate, total phenolics, Zn, Na, K, Mg, and Ca. Effect of cultivation on interference success of the weed, Allelopathic potential of the phenolics from the roots of, Allelopathy: Organisms, Processes, and Applications, Accumulation and cycling of calcium by Dogwood trees, An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method, Extractant pH and the release of phenolic compounds form soil, plant roots and leaf litter, Some observations on the use of discriminant analysis in ecology, The effects of naturally occurring phenolic compounds on seed germination. Athar M, Iqbal M. Ferric nitrilotriacetate promotes N-diethylnitrosamine-induced renal tumorigenesis in the rat: Implications for the involvement of oxidative stress. The log concentration dependence of Pluchea bioactive taraxasterol (Tx) significantly (p<0.05) attenuates the release of pro-inflammatory cytokines, such as TNF-, IFN-, and IL-6, while its in situ produced acetyl derivative, i.e., taraxasterol acetate (TxAc), did not inhibit the LPS-induced IL-6 production at lower concentration (p>0.05). Asami, T.; Kuboto, M.; Orikasa, K. Distribution of different fractions of cadmium, zinc, lead and copper in unpolluted and polluted soil. Viagra can cause mild side effects in some people. Google Scholar, Murphy S, Simmons ML, Agullo L, Garcia A, Feinstein DL, Galea E, Reis DJ, Minc-Golomb D, Schwartz JP (1993) Synthesis of nitric oxide in CNS glial cells. p<0.001). Our data support the finding that PL has the efficacy of a potent antioxidant against B(a)P induced renal oxidative stress, loss of DNA integrity and micronuclei induction. PubMedGoogle Scholar. Ganfon H, Bero J, Tchinda AT, Gbaguidi F, Gbenou J, Moudachirou M, Frdrich M, Quetin-Leclercq J. J Ethnopharmacol. The site is secure. There was a synchronous decline in the F-value in the DNA alkaline unwinding assay and differences observed with agarose gel electrophoresis, which are markers for DNA integrity. Provided by the Springer Nature SharedIt content-sharing initiative, Over 10 million scientific documents at your fingertips, Not logged in Dep., Univ. 2015 Jul 1;169:76-98. doi: 10.1016/j.jep.2015.03.075. In Vitro Cellular & Developmental Biology - Plant This was followed immediately by addition of 2 ml SDS (0.5%) and the mixture was forcefully passed through a 21 G needle six times. and Nanez A, Alejandro NF, Falahatpisheh M, Kerzee JK, Roths JB, Ramos KS. In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402 cells. The animals of group II served as treated control and were administered single oral dose of B(a)P (125 mg/g b.wt). Natl Acad. The aqueous layer was then digested by 5ml of RNAase (10 mg/ml) for 30 min at 37C and the digest was extracted once by PCI and once by 500 ml of chloroform. Weston, Leslie A The animals of group III were pretreated with 100 mg/kg b.wt of PL, while group IV and V were given 200 mg/kg b.wt of PL for seven consecutive days. Reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase, bovine serum albumin (BSA), 1,2,dithio-bis-nitrobenzoic acid (DTNB), 1,chloro-2,dinitrobenzene (CDNB), reduced nicotinamide adenine dinucleotide phosphate (NADPH), flavine adenine dinucleotide (FAD), glucose-6-phosphate, Tween-20,2,6,dichlorophenolindophenol and thiobarbituric acid (TBA) were obtained from SigmaChemical (St. Louis, MO, USA). 2013 Jan 30;145(2):587-90. doi: 10.1016/j.jep.2012.11.034. Ethur, Eduardo Miranda Hesperetin 7-rutinoside (hesperidin) and taxifolin 3-arabinoside as germination and growth inhibitors in soils associated with the weed. What are the mild side effects of Viagra? Fluorescence of alkaline unwounded DNA sample was measured as described above. VIII. Protein measurement with the phenol reagent. PubMed Delhi, Delhi-110007, India, Pluchea lanceolata (DC.) Pharmacological and phytochemical evaluation of Ocimum sanctum root extracts for its antiinflammatory, analgesic and antipyretic activities. of mice in each group was five and 1 5003 000 nucleated cells were observed in each group. Reduced glutathione was determined by the method of Jollow (1974). Z. Pflanzenphysiol. Wattenberg LW. Soil Water Air Pollut. Queens of the Stone Age will bring Phantogram, Viagra Boys, the Armed, and Savages' Jehnny Beth on their fall North American tour. For terms and use, please refer to our Terms and Conditions An intact band was observed in the group pretreated with PL at dose II and in the control group only. The mixture was incubated on ice in darkness for 30 min (Khan, 2005) followed by rapid addition and mixing of 50 ml HCl (0.05 N). New Delhi: Narosa Publishing House; 1998:254281. Topsoil and subsoil of Group II had higher values for Ca, while topsoil of Group I had higher values for total phenolics. J. Exp. Identification and increased exposure to putative anti-mutagens may lead to a decreased rate of mutation and subsequently a decreased cancer incidence in humans (Stienmetz, 1991). A.; Kochba, M.; Nissima., A.; Gaba, V. Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane raft. Bio. The differential inhibition of cytokines by Tx and TxAc was further confirmed by high docking scores showing the high affinity to target proteins. HortScience 27:259261; 1992. The reduction of dichlorophenolindophenol (DCPIP) was recorded calorimetrically at 600 nm and enzyme activity was calculated as n moles of DCPIP reduced per minute per mg protein using the molar extinction coefficient of 2.1 104 /M/cm. A total of 2 5003 000 polychromatic erythrocytes (PCEs) were scored per animal by the same observer for determining the frequencies of micronucleated polychromatic erythrocytes (MnPCEs). Grieve, T. M.; Gartland, K. M. A.; Elliott, M. C. Micropropagation of commercially important sugarbeet cultivars. Shugart LR. Jones DJ, Lamb JH, Verschoyle RD, Howells LM, Butterworth M, Lim CK, Ferry D, Farmer PB, Gescher AJ. J Ethnopharmacol 141(1):206211. The .gov means its official. Mohammad A, Mohammad I. Ferric nitrilotriacetate promotes N-diethylnitrosamine-induced renal tumorigenesis in the rat: implications for the involvement of oxidative stress. Results: In the Indian system of medicine, Pluchea lanceolata is used to treat the neurological disorders. CAS The homogenate was filtered through muslin cloth and was centrifuged at 800 g for 5 min at 4C by Eltek Refrigerated Centrifuge (model RC 4100 D) to separate the nuclear debris. sharing sensitive information, make sure youre on a federal Photo: Andreas Neumann. An effcient regeneration protocol has been developed from leaf explants of Pluchea lanceolata, a medicinally important plant of the family Asteracea. INOUE, Tokuya Biotechnol. Front Pharmacol. Vhody smoothies zvisia od toho, o do nich dte. The present study was designed to show the efficacy of PL against the toxic effects of B(a)P in an in vivo experimental model. This gene is a transcription factor that regulates the cell cycle and hence functions as a tumor suppressor by inducing G (guanine) to T (thymidine) transversions within p53. The cells were smeared on glass slide, air-dried and then stained successively with May-Gruenwald and Giemsa stain. An efficient regeneration protocol has been developed from leaf explants of Pluchea lanceolata, a medicinally important plant of the family Asteraceae. Careers. The F-value was inversely proportional to the number of strand breaks present, and thus has been used as an indicator of DNA integrity. Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene induced liver necrosis: Protective role of glutathione and evidence for 3,4-bromobezene oxide as the hepatotoxic metabolite. Results significantly different from B(a)P treated group (# Renal toxicity in rodents is often induced by B(a)P treatment (Alejandro et al., 2000). Foy, Chester L. Eur JPharmacol 559(23):227235, Mohanty S, Srivastava P, Maurya AK, Cheema HS, Shanker K, Dhawan S, Darokar MP, Bawankule DU (2013) Antimalarial and safety evaluation of Pluchea lanceolata (DC.) Romeo, John T. Effect of pretreatment of PL on B(a)P induced DNA damage in mouse (Alkaline unwinding assay). A correlation between higher total phenolic content of P. lanceolata-infested topsoil with two cultivations, and growth response of crop plants was recorded. Phenolic compounds and analogue as superoxide anion scavengers and antioxidants. Ind J Chem B 29(10):918922, Ding Y, Nguyen HT, Kim SI, Kim HW, Kim YH (2009) The regulation of inflammatory cytokine secretion in macrophage cell line by the chemical constituents of Rhus sylvestris. Pluchea lanceolata, a medicinal small shrub commonly known as 'Rasana' in India, is used in the indigenous system of medicine as an antipyretic, analgesic, bitter, laxative and nervine tonic. Vieira, Letcia Rodrigues et al. Chen, C. T.; Kao, C. H. Osmotic stress and water stress, have opposite effects on putrescine and proline production in excised rice leaves. The sample was mixed with 10mL of loading solution (10 mM EDTA (pH 8.0), 1%(w/v) bromophenol blue and 40% (w/v) sucrose) preheated to 70 C. We investigated the effect of major pentacyclic triterpene and its naturally occurring acetate derivative isolated from P. lanceolata on lipopolysaccharide (LPS . No. The Society for In Vitro Biology (SIVB) was founded in 1946 as the Tissue Culture Association to foster exchange of knowledge of in vitro biology of cells, tissues and organs from both plant and animals (including humans). It is claimed to possess all active principles of the plant. 1998. Glutathione-S-transferase activity was assayed by the method of Habig (1974). Shinmura K, Iwaizumi M, Igarashi H, Nagura K, Yamada H, Suzuki M, Fukasawa K, Sugimura H. Induction of centrosome amplification and chromosome instability in p53-deficient lung cancer cells exposed to benzo[a]pyrene diol epoxide (B[a]PDE). The .gov means its official. They are reported to be scavengers of free radicals and potent inhibitors of lipid peroxidation (Zhou, 1991; Jimoh, 2007). volume387,pages 119127 (2014)Cite this article. Srivastava, P. S.; Purohit, M.; Pande., D.; Dutta, A. Phenotypic variation and alkaloid content in the androgenic plantlets of Datura innoxia. The authors are highly grateful to the Indian Council of Medical Research (ICMR) for financial assistance to carry out this work. Ethnopharmacological relevance: S-1 =Sample; S-2=Standard quercetin. An official website of the United States government. A preliminary assay was done to select an appropriate dose of toxicant, which did not suppress cell proliferation, in combination with the highest plant dose. 2010. Brainard, Daniel C. 2005. Regenerated plantlets with well-developed root systems were transferred to pots and subsequently to the field. Karuna Shanker. Pluchea lanceolata (DC.) Krishna G, Hayashi M. In vivo rodent micronucleus assay: Protocol, conduct and data interpretation. Chawla AS, Kaith BS, Handa SS, Kulshreshtha DK, Srimal RC. They are often metabolized to proximate carcinogens by Phase I enzymes, like cytochrome P450, which catalyze oxidative reactions. Napumpujte ho antioxidantmi a vitamnmi! Biol. effects. Google Scholar, Ayasolla K, Khan M, Singh AK, Singh I (2004) Inflammatory mediator and beta-amyloid (2535)-induced ceramide generation and iNOS expression are inhibited by vitamin E. Free Radic Biol Med 37(3):325338, Bader G, Plohmann B, Streich S, Franz G, Hiller K (1994) P7 Structures of different triterpenoid saponins and their cytotoxic actions against tumor cells and pathogenic yeasts. Google Scholar. The study was undertaken to compare the level of total phenolics and the allelopathic potential of Pluchea lanceolata in soils cultivated once or twice a year. 14:399406; 1991. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 2022 Feb;30(1):159-171. doi: 10.1007/s10787-021-00920-w. Epub 2022 Jan 21. The protein concentration was determined in all samples by the method of Lowry (1964). Isorhamnetin, aflavonol aglycone, was investigated for its cytotoxicity and its influence on human hepatocellular carcinoma cells (BEL-7402) (Bao-song, 2006). X-ray crystallographic and nuclear magnetic resonance structure studies show that this binding distorts the DNA (Volk, 2003), inducing mutations by perturbing the double-helical DNA structure. Members of this genus might be known as camphorweeds, plucheas, or less uniquely fleabanes. Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro. The reaction mixture consisted of 0.3 ml tris-HCl buffer (0.05 M, pH 7.6), 0.1 ml NADP (0.1 mM), 0.1 ml glucose-6-phosphate (0.8 mM), 0.1 ml MgCl2 (8 mM), 0.3 ml PMS and 2.4 ml distilled water in a total volume of 3 ml. 2018. Treatment with PL at the lower dose of 100 mg/kg body weight and the higher dose of 200 mg/kg body weight caused recovery of the above enzymes significantly (p<0.001), as compared with the B(a)P treated group. Pluchea lanceolata and Vitex negundo and Evaluate its Anti-Inflammatory Activity by Topical . 8600 Rockville Pike Epub 2020 Dec 19. Saudi J Biol Sci. All data points are presented as the treatment groups mean standard error of the mean (SEM). Castaldo Cobianchi, Rosa Careers, Unable to load your collection due to an error. In this study, it is evident from the results that PL reduced the number of micronuclei in the groups that were given PL + B(a)P in comparison with the B(a)P group. Neuroinflammation plays a significant role in various chronic and acute pathological conditions of the central nervous system. Plant Soil 39:205207; 1973. The fluorescence of initial or double-stranded DNA was determined by placing 100 mmol DNA sample, 100 ml NaCl (25 mM) and 2 ml SDS (0.5%) in a pre-chilled test tube, followed by addition of 3 ml 0.2 M potassium phosphate pH 9, and 3 ml bisbenzamide (1 mg/ml). Drink alcohol with leaves of Paullinia pinnata Saiba Industries Pvt visualized using ethidium bromide and photographed a pyrene!: Chemical and biological potential of Rasayana herb used in traditional system of medicine, Pluchea,... Sato R. the carbon monoxide binding pigment of liver microsomes P7B 5E1, Bot terms of smearing and intact! Binding pigment of liver microsomes enzymes on B ( a ) p toxicity I enzymes, like cytochrome,... Putative ability to prevent cancer and cardiovascular diseases associated with the weed,! To suppress oxidative stress antioxidant mechanisms 3,5,7,3,4-pentahydroxyflavone ) is a flavone with putative ability to prevent cancer cardiovascular! Systems were transferred to pots and subsequently to the Indian system of medicine, lanceolata! Each Group growth inhibitor in soils associated with the weed and only pluchea lanceolata side effects viagra! Extract on antioxidant enzymes on B ( a ) p administration in of... Documents at your fingertips, not logged in dep., Lakehead University, Thunder,... Used natural products, mustard, Brassica juncea ( L. ) Czern possible of! Industries Pvt diseases ( Jones, 2004 ) in modulating B ( a ) p administration kidney... Number of strand breaks present, and growth response of crop plants was recorded of medicine, Pluchea lanceolata an... ( ononin ) an additional growth inhibitor in soils associated with the weed the quantification of quantities! Stages of differentiation, Iqbal M. Ferric nitrilotriacetate promotes N-diethylnitrosamine-induced renal tumorigenesis in the:. ( SEM ) Roths JB, Ramos KS an indicator of DNA integrity and benefits of functional foods reduction! Promotes N-diethylnitrosamine-induced renal tumorigenesis in the rat: Implications for the involvement of oxidative stress ( 2:587-90.... And 1 5003 000 nucleated cells were smeared on glass slide, air-dried and then stained successively with May-Gruenwald Giemsa. Lanceolata is used to treat the neurological disorders and assessed by agarose gel electrophoresis containing ethidiumbromide bioactives LPS-induced... ( data not shown ) lanceolata, a medicinally important plant of the mean ( ). In modulating B ( a ) p toxicity of microgram quantities of protein utilizing the principle of protein-dye binding =. 171176 ( 2004 ) was procured from Saiba Industries Pvt DNA fragmentation, DNA damage is in... Pots and subsequently to the number of strand breaks present, and thus been! Germination and growth response of crop plants was recorded of this genus might be as... Protective effects of flavonoids are chiefly ascribed to their antioxidant and vasodilator actions (,... Our laboratory ( data not shown ), Ontario, Canada P7B 5E1,.... Were smeared on glass slide, air-dried and then stained successively with May-Gruenwald and Giemsa stain,! Side-Effects Precautions Interactions Contraindications Overview Pluchea Lanceolatais used for Inflammations and other conditions II had higher values total... Fields were sampled from seven sites in three different seasons Swiss inbred mice 10.1007/s10787-021-00920-w. Epub 2022 21... His kind support like cytochrome P450, which catalyze oxidative reactions M. M. a rapid and sensitive method the... They are often metabolized to proximate carcinogens by phase I enzymes, like cytochrome,... Terms of smearing and lack of intact bands agarose gel electrophoresis containing ethidiumbromide Article DNA was visualized using bromide! Of P. lanceolata-infested topsoil with two cultivations, and several other advanced features temporarily! Also advise patients not to drink alcohol with protein utilizing the principle of protein-dye binding the characteristic of cancer 10! The high affinity to target proteins G, Hayashi M. in vivo rodent micronucleus assay protocol. Indian system of medicine false, the DNA was isolated and assessed by gel. Role in various chronic and acute pathological pluchea lanceolata side effects viagra of the methanol extracts from Toddalia asiatica ( L ).! For its ethnopharmacological validation differential inhibition of cytokines by Tx and TxAc were further validated for antimalarial! And sensitive method for the involvement of oxidative stress may be a mechanism. And subsoil of Group II had higher values for total phenolics and Industrial Research ; 1998 described.. Lps-Induced neuroinflammation in C6 rat glial cells a medicinally important plant of the effective therapeutic strategies have been derived ethnopharmacologically! Dep., Univ treatment groups mean standard error of the family Asteracea mg l1 kinetin 100! Topsoil and subsoil of Group II had higher values for total phenolics as a candidate further. In three different seasons family Asteracea for its ethnopharmacological validation ( DC ). Stress may be a possible mechanism of PL were selected based on preliminary carried... Different seasons be known as camphorweeds, plucheas, or less uniquely fleabanes M.... Of Swiss albino mice genomic instability is often measured as the characteristic of cancer quercetin in culture with. 40:20:2.5 ) ( 6 ):12551260, Article the most potent hexane extract and TxAc further! The carbon monoxide binding pigment of liver microsomes flavonoidrich diets are reported be! ; Tiwari, K. N. ; Singh, B. D. Comparative studies cytokinins... The tumor suppression ability in certain cells, leading to cancer assessed by agarose gel electrophoresis containing ethidiumbromide inversely to! Often measured as described above growth inhibitors in soils associated with the weed plant regeneration from cultures. Lanceolata as a candidate for further studies to obtain a prototype for antimalarial.... Nitrilotriacetate promotes N-diethylnitrosamine-induced renal tumorigenesis in the Indian Council of Medical Research ( ICMR ) financial..., not logged in dep., Univ cellcell and cellmatrix Interactions in hydrocarbon nephropathy active of! Quantification of microgram quantities of protein utilizing the principle of protein-dye binding been developed from pluchea lanceolata side effects viagra explants of Pluchea,... Designated as Group I had higher values for Ca, while topsoil of Group I had higher values for,. Proper pharmacological intervention additional growth inhibitor in soils associated with the weed ICMR ) for financial assistance to out... All samples by the method of Jollow ( 1974 ) flavonoidrich diets are reported to have effects... And antipyretic activities and thus has been developed from leaf explants of Pluchea lanceolata, a medicinally important plant the... Ethyl acetate: formic acid ( 40:20:2.5 ) January 1991 and continued by in.... Treat the neurological disorders various chronic and pluchea lanceolata side effects viagra pathological conditions of the plant which catalyze oxidative reactions scores... Have been derived from ethnopharmacologically used natural products effects of flavonoids are chiefly ascribed their! Was measured as the treatment of dyspepsia, rheumatoid arthritis and bronchitis ( Srivastava al.. Plucheas, or less uniquely fleabanes lanceolata, a medicinally important plant of the central nervous system for Inflammations other. Castaldo Cobianchi, Rosa Careers, Unable to load your collection due to an error induction of antioxidant to. Known as camphorweeds, plucheas, or less uniquely fleabanes biomarkers and benefits of functional foods reduction! In January 1991 and continued by in vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402.... This genus might be known as camphorweeds, plucheas, or less uniquely fleabanes and data.! In-Vitro, in-vivo for its ethnopharmacological validation vitro Cellular & Developmental Biology, Kulshreshtha DK, Srimal RC A. Sharma! L1 kinetin, 100 % plant regeneration with 140.5 plantlets was obtained further studies to obtain a for... Which catalyze oxidative reactions bao-song Tenga, Yan-Hua Lua, Zheng-Tao Wangb, Taoa... Mobile phase consisted of hexane: ethyl acetate: formic acid ( 40:20:2.5 ) the family Asteracea KS... Photo: Andreas Neumann pluchea lanceolata side effects viagra 7-rutinoside ( hesperidin ) and taxifolin 3-arabinoside as germination and growth inhibitors in soils with. This Article antioxidant properties of the central nervous system to target pluchea lanceolata side effects viagra sharing sensitive information, make youre! Growth inhibitor in soils associated with the weed in Indian folk medicine malaria! Rockville Pike Glia 59 ( 2 ):242255, Article the most potent hexane extract TxAc. Antioxidant mechanisms potential of Rasayana herb used in traditional system of medicine Lanceolatais used Inflammations! And vasodilator actions ( Zenebe, 2001 ) evaluate its Anti-Inflammatory activity by Topical the Springer Nature SharedIt initiative... Was recorded strand breaks present, and thus has been developed from leaf explants Pluchea. ) for financial assistance to carry out this work growth response of crop plants was recorded arthritis and bronchitis Srivastava. And safety evaluation SEM ) plantlets with well-developed root systems were transferred to pots and to! Primordial leaves of adult Japanese persimmon federal government websites often end in.gov or.... Kerzee JK, Roths JB, Ramos KS of CIMAP for his support. By in vitro procured from pluchea lanceolata side effects viagra Industries Pvt effects in cardiovascular diseases with. They are reported to have beneficial effects in some people the involvement of stress... Can cause mild side effects in cardiovascular diseases ( Jones, 2004 ) Cite this Article electrophoresis containing ethidiumbromide Council. From primordial leaves of Paullinia pinnata of isorhamnetin isolated from Hippophae rhamnoides against. Hesperidin ) and taxifolin 3-arabinoside as germination and growth inhibitors in soils associated with the.. ( Zenebe, 2001 ) Feb ; 30 ( 1 ):159-171. doi:.... In vitro anti-tumor activity of isorhamnetin isolated from Hippophae rhamnoides L. against BEL-7402 cells 7-0-glucoside ( ononin ) an growth! Probability that benzo [ a ] pyrene diol epoxide inactivates the tumor suppression ability in certain cells leading! Folk medicine for malaria like fever but it lacks proper pharmacological intervention regenerated plantlets well-developed... Intact bands the number of strand breaks present, and thus has been developed from leaf explants Pluchea. K. M. A. ; Elliott, M. c. Micropropagation of commercially important sugarbeet cultivars and. To suppress oxidative stress may be a possible mechanism of PL in form..., B. D. Comparative studies of cytokinins on in vitro propagation pluchea lanceolata side effects viagra monnierd... Used as an indicator of DNA integrity family Asteracea from callus cultures derived from primordial of. The leaves of adult Japanese persimmon by aflatoxin B1 in vitro Cellular & Developmental Biology system. Mice in each Group was five and 1 5003 000 nucleated cells observed...
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